Dengucheck WB
Rapid test for the detection of IgM & IgG antibodies to Dengue Virus
Application
Denguecheck WB is rapid, qualitative immunochromatographic test for simultaneous detection of IgM & IgG antibodies to Dengue virus in human serum/plasma/ whole blood. The test system can be used for screening of Dengue viral infection and as an aid for differential diagnosis of the self limiting primary Dengue infections and the potentially fatal secondary Dengue infections in conjunction with other criteria.
Principle
Dengucheck WB utilizes the principle of agglutination of antibodies/ antisera with respective antigen in immuno- chromatography format along with use of nano gold particles as agglutination revealing agent. Specific Agglutinating sera for Human IgM and Agglutinating sera for Human IgG are immobilized on the nitrocellulose membrane as two individual test bands (IgM and IgG) in the test device at region ‘M’ and region ‘G’ respectively. The IgG band is closer to the sample well and the IgM band is close to the control area marked ‘C’.
As the test sample flows through the membrane assembly within the test device, the colored–Dengue specific antigen-colloidal gold conjugate complexes with specific antibodies (IgM and IgG) to Dengue virus, if present in the sample. This complex moves further on the membrane to the test region where it is immobilized by the specific Agglutinating sera for Human IgG and/or Agglutinating sera for Human IgM coated on the membrane leading to formation of a colored band which confirms a positive test result. Absence of these colored bands in the test region indicates a negative test result.
A built-in control band in the control area marked ‘C’ appears when the test has been performed correctly, regardless of the presence or absence of anti-Dengue virus antibodies in the specimen and serves to validate the test performance
Reagents
A. Individual pouches, each containing:
- Membrane assembly pre-dispensed with Dengue virus specific antigen colloidal gold conjugate, streptavidin gold conjugate, Agglutinating sera for Human IgM at test region ‘M’, Agglutinating sera for Human IgG at test region ‘G’ and Biotinylated BSA at control region ‘C’.
- Desiccant pouch.
B. Sample running buffer in a dropper bottle
C. Package Insert
Strorage / Stability
The test kit (including sealed pouches) may be stored between 4ºC to 30ºC till the duration of the shelf life as indicated on the pouch / carton. DO NOT FREEZE.
After first opening of the sample running buffer bottle, it can be stored between 4ºC to 30ºC for the remaining duration of its shelf life.